Short Description
Measures peroxidase activity using a colorimetric method.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Peroxidase Assay Kit provides a sensitive colorimetric method for quantifying peroxidase activity in biological samples and experimental matrices. The system utilizes a specific substrate that produces a colored product upon oxidation, allowing for precise kinetic or endpoint measurements. This versatile tool is ideal for researchers investigating oxidative defense, cell signaling, and enzymatic regulation, offering high-throughput compatibility and consistent performance in biochemical studies.
Features
Utilizes a sensitive chromogenic substrate for the rapid quantification of peroxidase activity, facilitating high-throughput screening of oxidative stress markers and enzymatic research across various models.
Process Relevance
Quantifying peroxidase activity to assess enzymatic defense against peroxides and optimize oxidative stress models.
Application Stage
Enzyme characterization and oxidative stress research.
Applications
Determine peroxidase enzymatic activity in biological extracts and fluids to evaluate oxidative metabolism and enzyme kinetics in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, cell culture supernatants.
Research Areas
Enzyme Kinetics; Plant Biology; Oxidative Stress
Datasheet