GuardianOx™ Peroxidase Assay Kit

CAT#: ITS-1125-YL36
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Measures peroxidase activity using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Peroxidase Assay Kit provides a sensitive colorimetric method for quantifying peroxidase activity in biological samples and experimental matrices. The system utilizes a specific substrate that produces a colored product upon oxidation, allowing for precise kinetic or endpoint measurements. This versatile tool is ideal for researchers investigating oxidative defense, cell signaling, and enzymatic regulation, offering high-throughput compatibility and consistent performance in biochemical studies.
Features
Utilizes a sensitive chromogenic substrate for the rapid quantification of peroxidase activity, facilitating high-throughput screening of oxidative stress markers and enzymatic research across various models.
Process Relevance
Quantifying peroxidase activity to assess enzymatic defense against peroxides and optimize oxidative stress models.
Application Stage
Enzyme characterization and oxidative stress research.
Applications
Determine peroxidase enzymatic activity in biological extracts and fluids to evaluate oxidative metabolism and enzyme kinetics in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, cell culture supernatants.
Research Areas
Enzyme Kinetics; Plant Biology; Oxidative Stress
Size
100 tests
Data Output Type
Quantitative enzyme activity measurement (kinetic)
Assay Time
20 min
LOD
LOD: OD, FL: 4, 0.8 U/L
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
These enzymes catalyze the oxidation of various substrates using hydrogen peroxide as an electron acceptor. They are involved in numerous biological processes, including the defense against pathogens and the synthesis of structural components.
Pathways
These metals act as essential cofactors for numerous enzymes in pathways ranging from oxygen transport (Iron/Heme) to redox catalysis (Copper/Superoxide Dismutase). They serve as upstream regulators of enzyme activity and cellular metal-trapping proteins.
For Research Use Only | Not For Clinical Use
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