Gut Microbiota Metabolomics Service

The gut microbiota produces plentiful metabolite, which plays a vital role in microbe selection and the construction of a metabolic signaling network. Moreover, these metabolite profiles have a great influence on lifestyle and diet which are vital factors for chronic and acute diseases. Creative Biolabs offers gut microbiota metabolomics services to help you reveal how diverse microorganisms influence health and disease.

Gut Microbiota Metabolome

The gut microbiota is exclusively responsible for several metabolic important functions, including vitamin and short-chain fatty acid (SCFAs) production, amino acid (AAs) synthesis, bile acid biotransformation, hydrolysis and fermentation of non-digestible substrates. The beneficial effects of gut microbiota include epithelial homeostasis, immune-cell homeostasis and development (Th1 vs. Th2 and Th17), food digestion, enteric nerve regulation, support of angiogenesis, and fat metabolism. However, when the balance between the resident microbiota and the gastrointestinal (GI) tract is disrupted, intestinal and extraintestinal diseases can be developed, including allergy, obesity, inflammatory bowel disease (IBD), cancer, diabetes, cardiovascular dyslipidemia, and neuropathology.

Fig.1 Effect of gut microbiota metabolome on organs and tissues. (Vernocchi, 2016)

Metabolomics approach has been applied to the gut microbiota study that focused on the exploration of disease-related metabolites to obtain detailed information on the gut metabolic pathways. Moreover, metabolomics represents an unprecedented approach for collecting the complex metabolic interactions between the host and its commensal microbial partners, offering the opportunity to define individual and population phenotypes.

Applications of Metabolomics

• Define the metabolic profile
• Identify and quantify classes and compounds of interest
• Characterize small molecules produced by intestinal microbes
• Define the biochemical pathways of metabolites

Workflow of Gut Microbiota Metabolomics

A metabolomics analysis typically comprises the following 3 steps:

Fig.2 General workflow of metabolomics analysis.

Our Services

• Detection Method
  Creative Biolabs has developed a range of analytical platforms to help you facilitate your gut microbiota metabolomics program:
• Gas chromatography (GC)
• Liquid chromatography (LC)
• High-pressure LC (HPLC)
• Ultra performance LC (UPLC)
• Fourier transform infrared spectroscopy (FTIR)
• Ion cyclotron resonance-FT (ICR-FT)
• Capillary electrophoresis (CE) coupled with mass spectrometry (MS)
• Nuclear and proton nuclear magnetic resonance spectroscopy (NMR-¹H-NMR)
• Data Analysis
  Creative Biolabs provides biostatistics and mathematical approaches for complex metabolic profiling.
• Genetics
• Environmental factors
• Gut microbiota activity
• Lifestyle
• Eating habits

Sample Type

• Feces
• Urine
• Plasma/serum
• Saliva
• Exhaled breaths
• Cerebrospinal fluid
• Stool and intestinal contents
• Tissues from target organs

If you are interested in our gut microbiota metabolomics services, please contact us for any discussion about your microbiome project service needs.

Reference

1. Vernocchi, P.; et al. Gut microbiota profiling: metabolomics based approach to unravel compounds affecting human health. Front Microbiol. 2016, 7: 1144.

For Research Use Only | Not For Clinical Use