The High Content Analysis (HCA)-based Low-Density Lipoproteins (LDL) Uptake Assay is a quantitative platform that utilizes advanced imaging techniques to assess and analyze cellular uptake of LDL particles in a high-throughput manner, providing insights into lipid metabolism and cellular health.

Why Do LDL Uptake

LDL uptake is a crucial physiological process for maintaining cellular function and lipid homeostasis. LDL particles serve as carriers of cholesterol and triglycerides, delivering these essential lipids to cells throughout the body. Cells uptake LDL via specific receptors, primarily the LDL receptor, which facilitates the internalization of LDL particles into the cytoplasm. This process is vital for synthesizing cellular membranes, hormones, and bile acids, all of which rely on cholesterol. Additionally, adequate LDL uptake helps ensure that cells have sufficient energy reserves and structural components. However, excessive LDL uptake and accumulation can lead to pathologies such as atherosclerosis, highlighting the importance of regulatory mechanisms governing LDL metabolism.

HCA-based LDL Uptake Assay at Creative Biolabs

Our service offers a sophisticated platform for assessing cellular uptake of LDL, a crucial factor in understanding lipid metabolism and cardiovascular health. Utilizing advanced imaging technologies, this service quantitatively evaluates the internalization of fluorescently labeled LDL particles in various cell types under diverse experimental conditions. The HCA system provides high-resolution images and automated quantification, enabling customers to analyze multiple parameters such as uptake efficiency, subcellular localization, and cytotoxic effects in a high-throughput manner.

• Assay Information

• Process of Our Service

  

  Cell Preparation

  Cells of interest (e.g., fibroblasts or macrophages) are cultured in suitable conditions and plated onto multi-well plates for analysis.

  LDL Labeling

  LDL particles are typically labeled with a fluorescent dye to enable visualization. This labeling allows the tracking of LDL uptake through fluorescence microscopy.

  Incubation

  The labeled LDL is added to the cells and incubated for a specific time period at 37°C, allowing the cells to internalize the LDL particles.

  Washing

  After incubation, unbound LDL is removed by washing the cells with a buffer solution, which reduces background fluorescence and ensures that only internalized LDL is measured.

  Imaging

  The cells are imaged using a high-content imaging system, which captures multiple fields of view, allowing for the quantification of fluorescent signals within individual cells.

  Data Analysis

  The high-content imaging software analyzes the images to quantify parameters such as the intensity of fluorescence (indicative of LDL uptake), cell number, and cellular distribution of the LDL. This is done by applying algorithms for segmentation and quantification.

Frequently Asked Question

Q1: What are the applications of HCA-based LDL uptake assay?

A1: The HCA-based LDL uptake assay is a valuable tool in both research and clinical settings for studying lipid metabolism and cardiovascular health. Applications include investigating the effects of genetic modifications, pharmacological agents, or dietary changes on LDL metabolism, as well as screening for potential therapeutic compounds that may attenuate atherosclerosis. Furthermore, the assay can help in understanding the role of LDL in conditions like diabetes and obesity, offering insights into disease mechanisms and potential interventions for managing cardiovascular risk.

Contact Us

At Creative Biolabs, we specialize in advanced diagnostic solutions, including our proprietary HCA-based LDL Uptake Assay. Our innovative assay is designed to provide accurate and reliable measurements of low-density lipoprotein uptake, facilitating an enhanced understanding of lipid metabolism and cardiovascular health. Our experienced team is committed to supporting your research needs with top-notch service and expertise. If you have any questions or would like to learn more about how our HCA-based LDL Uptake Assay can benefit your work, please don't hesitate to reach out. We look forward to assisting you!

For Research Use Only | Not For Clinical Use