Short Decsription
ELISA kit for the measurement of CHO host cell proteins.
Description
Detection kit for the determination of Hela host cell protein contamination bulk products. Based on the "sandwich"ELISA methods, it with quick and high sensitivity analysis.
Features
Detect and quantify HCP concentrations at any point in your purification process;
ELISA-based: quick, familiar format with high sensitivity;
96-well plate coated with capture antibody;
HCP standards, reporting antibody, streptavidin-HRP conjugate, TMB substrate, buffers included;
Applications
Host Cell Proteins Detection
Assay Measures
The detection kit is based off of "sandwich"ELISA methods. Host Cell Protein antibodies are immobilized to 96 well plates. Samples are added, and Host Cell Proteins are bound by the antibodies. Biotinylated Host Cell Protein antibody is then added, followed by strepavidin HRP, which binds to biotin. TMB substrate is then added, and HRP catalyzes the conversion of TMB to a colored product. The reaction is then stopped and read on a standard colorimetric plate reader.