HCT-116 In Vitro Molecular Biology Assay (Angiogenesis)

This assay is to provide HCT-116-based In Vitro Molecular Biology Assay (Angiogenesis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HCT-116

Human

HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Molecular Biology Assay (Angiogenesis)

HCT-116-cell based In Vitro Molecular Biology Assay (Angiogenesis)

Direct detection methods of factors effecting angiogenesis mainly include real-time PCR, western blotting and enzyme-linked immunosorbent assay (ELISA). JAG1, PECAM1, ANGPTL3, MMP2, MMP9, HAND2, TGFA, LAMA5, IL1B, FIGF, TIMP3, ANGPT1, LECT1, EREG and ANPEP are the key genes reported to control angiogenesis.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).