HCT-8 In Vitro BrdU Assay (Cell Proliferation)

CAT#: ITS-1122-YF119
Target Cell Organism: Human
Target Cell Alternative Name: HRT-18
Target Cell Name: HCT-8
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HCT-8-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HCT-8
Target Cell Organism
Human
Target Cell Background
HCT-8 [HRT-18] cells were isolated from the large intestine of a 67-year-old, male, adenocarcinoma patient. HCT-8 [HRT-18] are used for cancer and toxicology research.
Target Cell Alternative Name
HRT-18
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Short Description
HCT-8-cell based In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent sig nal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Proliferation Assay
Assay MOA
Cell-based Assays Based on DNA Synthesis
For Research Use Only | Not For Clinical Use
Online Inquiry