Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HEL-based In Vitro ATP Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HEL
Target Cell Organism
Human
Target Cell Background
A new human erythroleukemia cell line has been established. This line, designated HEL, is capable of spontaneous and induced globin synthesis, producing mainly Gγ and Aγ chains. Embryonic chains (ε, ζ) and α chains are detectable in very small amounts; β chains are undetectable. This line provides a new model system for studying aspects of erythroid cell differentiation and differential globin gene expression.
Related Diseases
Erythroleukemia
Research Area
Oncology
Assay Name
In Vitro ATP Assay (Cell Viability)
Short Description
HEL-cell based In Vitro ATP Assay (Cell Viability)
Assay Description
Adenosine triphosphate (ATP) assay is another widely used cell viability assay. When the cancer cells undergo apoptosis or necrosis upon treatment with a compound, cellular ATP content decreases and it can be quantified by colorimetric, fluorometric or luminometric way. The latest bioluminescence assays measure cellular ATP content using the enzyme luciferase which is more sensitive and reproducible when determining cell viability compared to conventional assays.
Assay Alternative Names
Adenosine Triphosphate Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins