HEL In Vitro Representational Difference Analysis (RDA) Assay

This assay is to provide HEL-based In Vitro Representational Difference Analysis (RDA) Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HEL

Human

A new human erythroleukemia cell line has been established. This line, designated HEL, is capable of spontaneous and induced globin synthesis, producing mainly Gγ and Aγ chains. Embryonic chains (ε, ζ) and α chains are detectable in very small amounts; β chains are undetectable. This line provides a new model system for studying aspects of erythroid cell differentiation and differential globin gene expression.

Erythroleukemia

Oncology

In Vitro Representational Difference Analysis (RDA) Assay

HEL-cell based In Vitro Representational Difference Analysis (RDA) Assay

This technique can detect sequence differences of two different DNA segments. In representational difference analysis, genomes to be compared are digested by specific restriction enzymes and ligated to specific oligonucleotide linkers, providing two different representations of the two genomes to be generated by PCR with primers specific for oligonucleotide linkers. Following the generation of amplicons, oligonucleotide linkers are removed and a new linker is attached only to test amplicons. These amplicons (tester) are then mixed with driver amplicons, which lack linkers. Amplification only occurs with DNA fragments specific to the tester (with primers specific for linkers).

Genome Alteration Assays

Aberrant or somatic mutations are more commonly found in the DNA of cancer cells compared to normal cells. There is an equilibrium that exists between DNA damage and repair in normal cells. However, in cancer cells these events are disturbed, resulting in mutations and genomic instability. Genomic instability in cancer cells causes chromosomal aberrations, microsatellite instability, aneuploidy and uncontrolled gene amplifications and genetic instability in cancer cells are mainly due to point mutations or chromosomal aberrations such as insertions, deletions and translocation, resulting in mutated proteins.