Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Hep55.1c-based In Vitro WST Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Hep55.1c
Target Cell Organism
Mouse
Target Cell Background
The Hep55.1C cell line is derived from carcinogen-induced liver tumors of C57BL/6 mice and utilized in a syngeneic orthotopic murine model of HCC.
Related Diseases
Hepatocellular Carcinoma
Research Area
Oncology
Assay Name
In Vitro WST Assay (Cell Viability)
Assay Description
Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins