Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Hepa 1-6-based In Vitro WST Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Hepa 1-6
Target Cell Organism
Mouse
Target Cell Background
The Hepa 1-6 tumorigenic epithelial cell line is derived from liver cells of the BW7756 mouse (Mus musculus) with hepatoma. Hepa 1-6 has been negatively tested for mousepox and is widely used in mammalian liver tissue functions research.
Target Cell Alternative Name
Hepa1-6
Related Diseases
Liver Hepatoma
Research Area
Oncology
Assay Name
In Vitro WST Assay (Cell Viability)
Short Description
Hepa 1-6-cell based In Vitro WST Assay (Cell Viability)
Assay Description
Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also generate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins