HepG2 In Vitro Calcium Concentration-based Apoptosis Assay

This assay is to provide HepG2-based In Vitro Calcium Concentration-based Apoptosis Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HepG2

Human

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Hep G2

Hepatocellular Carcinoma

Oncology

In Vitro Calcium Concentration-based Apoptosis Assay

HepG2-cell based In Vitro Calcium Concentration-based Apoptosis Assay

The endoplasmic reticulum (ER) plays an important role in the storage and maintenance of Calcium. Release of stored Calcium from the ER and uptake by the mitochondria are activated in apoptosis. Measurement of cytosolic Calcium concentrations as an apoptotic signal has been reported in studies.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Measurement of Cytosolic Ca2+ Concentration Assay