HepG2 In Vitro Lactate Assay (Angiogenesis)

This assay is to provide HepG2-based In Vitro Lactate Assay (Angiogenesis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HepG2

Human

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Hep G2

Hepatocellular Carcinoma

Oncology

In Vitro Lactate Assay (Angiogenesis)

HepG2-cell based In Vitro Lactate Assay (Angiogenesis)

Accumulation of lactate in cancer cells has been linked with poor survival and cancer metastasis. Higher glucose uptake in cancer cells is considered as the main cause for the accumulation and secretion of lactate by cancer cells. The lactate/pyruvate ratio is an essential parameter for the maintenance of the redox state of cells. Therefore, evaluation of cellular lactate levels allows assessment of the cellular oxygen utilization and demand. A number of commercially available colorimetric or fluorometric kits have been developed to detect lactate levels in cells and tissues.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).