HepG2 In Vitro SRB Assay (Cell Viability)

CAT#: ITS-1022-YF1163
Target Cell Organism: Human
Target Cell Alternative Name: Hep G2
Target Cell Name: HepG2
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HepG2-based In Vitro SRB Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HepG2
Target Cell Organism
Human
Target Cell Background
Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
Target Cell Alternative Name
Hep G2
Related Diseases
Hepatocellular Carcinoma
Research Area
Oncology
Assay Name
In Vitro SRB Assay (Cell Viability)
Short Description
HepG2-cell based In Vitro SRB Assay (Cell Viability)
Assay Description
The sulforhodamine B is another common colorimetric assay that is used to investigate viability of cancer cells. The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500-540 nm.
Assay Alternative Names
Sulforhodamine B Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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