HL-60 In Vitro WST Assay (Cell Viability)

This assay is to provide HL-60-based In Vitro WST Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HL-60

Human

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center.

HL60

Leukemia

Oncology

In Vitro WST Assay (Cell Viability)

HL-60-cell based In Vitro WST Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins