HT1080 In Vitro MTT Assay (Cell Viability)

CAT#: ITS-1022-YF1367
Target Cell Organism: Human
Target Cell Alternative Name: HT 1080
Target Cell Name: HT1080
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide HT1080-based In Vitro MTT Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
HT1080
Target Cell Organism
Human
Target Cell Background
HT-1080 human cells are epithelial cells derived from connective tissue from a patient with Fibrosarcoma that can be used in research.
Target Cell Alternative Name
HT 1080
Related Diseases
Fibrosarcoma
Research Area
Oncology
Assay Name
In Vitro MTT Assay (Cell Viability)
Short Description
HT1080-cell based In Vitro MTT Assay (Cell Viability)
Assay Description
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay is one of most commonly used assays for cancer drug screening. This assay has been very popular among cancer researchers as an initial assay in drug discovery. NAD(P)H-dependent oxidoreductase or dehydrogenases in viable cells can reduce MTT into purple-colored formazan, which can then be solubilized for spectrophotometric analysis.
Assay Alternative Names
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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