Human ARG1 ELISA Kit, Lot 21AO-1053 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P964
Product Type: ELISA Kit
Target: ARG1
Short Description
The kit is designed for in vitro quantitative measurement of Human ARG1 in Ascitic Fluid, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human arginase-1 (ARG1) concentrations in serum, plasma, ascitic fluid, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
ARG1
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Ascitic Fluid, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human ARG1. No significant cross-reactivity or interference between human ARG1 and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human ARG1 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
0.39 ng/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
Expiry Date
6 months
Note
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
Restrictions
For Research Use only
Alternative Name
Arginase I
Synonyms
SI:zC146F4.4 (novel protein with NUDIX domain); si:ch211-146f4.3; argi1; AI; AI256583; Arg-1; PGIF; arginase 1; arginase; Arginase-1; arginase; liver; L-arginase; arg1; PGTG_16455; argi1; ARG1; Arg1
Background
Abbreviation: ARG1
Alias: arginase 1|liver-type arginase|type I arginase
Gene ID
383
UniProt
P05089
Pathways
Cellular Response to Molecule of Bacterial Origin
For Research Use Only | Not For Clinical Use
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