Human ERBB3/HER3 Sandwich ELISA Kit-4 pg/mL

This product is a 96-well enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of human ERBB3/HER3

ERBB3/HER3 detection

ERBB3/HER3

Human

Colorimetric

Quantitative

Cell culture supernatants, plasma, serum

96-well plates

Epidermal growth factor receptor (EGFR/ERBB) family

Certificate of quality

Pre-coated 96-well strip microplate; Wash buffer; Stop solution; Assay diluent(s); Lyophilized standard; Biotinylated detection antibody; Streptavidin-conjugated HRP; TMB one-step substrate

4 pg/mL

4 pg/mL-2500 pg/mL

1, 2, or 5

1.Distilled or deionized water 2.Precision pipettes to deliver 2 µL to 1 µL volumes 3.Adjustable 1-25 µL pipettes for reagent preparation 4.100 µL and 1 liter graduated cylinders 5.Tubes to prepare standard and sample dilutions 6.Absorbent paper 7.Microplate reader capable of measuring absorbance at 450nm 8.Log-log graph paper or computer and software for ELISA data analysis

1.Prepare all reagents, samples and standards as instructed in the manual; 2.Add 100 µL of standard or sample to each well; 3.Incubate 2.5 h at RT or O/N at 4°C; 4.Add 100 µL of prepared biotin antibody to each well; 5.Incubate 1 h at RT; 6.Add 100 µL of prepared Streptavidin solution to each well; 7.Incubate 45 min at RT; 8.Add 100 µL of TMB One-Step Substrate Reagent to each well; 9.Incubate 30 min at RT; 10.Add 50 µL of Stop Solution to each well; 11.Read at 450 nm immediately.

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.

P21860

Stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage: -80°C.

For research use only. Intended for use by laboratory professionals.

ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3

ERBB3; C-erbB-3; C-erbB3; ErbB-3; HER3; p85-sErbB3; p45-sErbB3; MDA-BF-1; ErbB3-S; LCCS2; p180-ErbB3

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene.

2065