Human NFAT-Luciferase Reporter Recombinant Cell Line, Jurkat (CAT#: IOC-LX157)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. This cell line has been validated for response to thapsigargin, ionomycin, phorbol 12-myristate 13-acetate (PMA), and anti-CD3 Ab, and has also been validated for T cell activation. Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

• Monitor intracellular calcium levels.
• Screen for activators or inhibitors of PKC/ Ca2+ pathway.
• Screen for agonists or antagonists of T cell receptors and co-inhibitors.
• Control for immune checkpoint NFAT reporter cell lines.

Human

Jurkat

NFAT-Luciferase Reporter

The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.