Human TIMD4 ELISA Kit, Lot 21AO-1179 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human TIMD4 in Plasma, Cell Culture Supernatant, Serum.

Human TIM-4 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

ELISA

TIMD4

Human

Colorimetric

Sandwich ELISA

Quantitative

Plasma, Cell Culture Supernatant, Serum

This ELISA antibody pair recognizes Human TIM-4.

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

100 μL

Pre-coated

-20 °C

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

6 months

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

For Research Use only

T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4)

RGD1564516; MGC99322; TIMD4; B430010N18Rik; TIM-4; Tim4; SMUCKLER; TIM4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing 4 L homeolog; T cell immunoglobulin and mucin domain containing 4; Timd4; TIMD4; timd4.L

91937

Q96H15

Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.