Human VEGF ELISA Kit, Lot 21AO-941 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P852)

The kit is designed for in vitro quantitative measurement of Human VEGF in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.

For quantitative detection of VEGF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

ELISA

Anti-VEGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VEGF polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of VEGF can be calculated.

VEGF

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum, Tissue Lysate