Human VEGF ELISA Kit, Lot 21AO-941 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human VEGF in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.

For quantitative detection of VEGF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

ELISA

Anti-VEGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VEGF polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of VEGF can be calculated.

VEGF

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

1. One 96-well plate pre-coated with anti-Human VEGF antibody 2. Lyophilized Human VEGF standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human VEGF antibody (Concentrated): 130 µl.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

< 1 pg/mL

Pre-coated

1.Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2.It is recommend to measure each standard and sample in duplicate. 3.Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4.Do not reuse pipette tips and tubes to avoid cross contamination.

For Research Use only

Vascular Endothelial Cell Growth Factor

MVCD1; VEGF; VPF; Vegf; Vegf120; Vegf164; Vegf188; Vpf; VEGF-A; VEGF164; eVEGF120; eVEGF165; vegf-a; vegf; vascular endothelial growth factor A; vascular endothelial growth factor A L homeolog; vascular endothelial growth factor; vascular endothelial grow

Vascular endothelial growth factor (VEGF) is encoded by VEGF gene, which contains 8 exons and maps to 6p21.3. It is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis.

7422

P15692

RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling