Human VTCN1 ELISA Kit, Lot 21AO-1143 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1054
Product Type: ELISA Kit
Target: VTCN1
Short Description
The kit is designed for in vitro quantitative measurement of Human VTCN1 in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human B7-H4 concentrations in serum, plasma, cell culture supernates, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
VTCN1
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human B7-H4. No significant cross-reactivity or interference between human B7-H4 and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human B7-H4 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
1.95 ng/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C.
Restrictions
For Research Use only
Alternative Name
V-Set Domain Containing T-Cell Activation Inhibitor 1/Costimulatory molecules B7H4
Synonyms
B7h4; B7s1; B7x; BC032925; B7-H4; B7H4; B7S1; B7X; B7h.5; PRO1291; VCTN1; RGD1311204; V set domain containing T cell activation inhibitor 1 L homeolog; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; V set domain containing T cell activation inhibitor 1; vtcn1.L; VTCN1; vtcn1; Vtcn1
Background
V-set domain-containing T-cell activation inhibitor 1 (VTCN1) negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity.
Gene ID
79679
UniProt
Q7Z7D3
For Research Use Only | Not For Clinical Use
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