The Annexin V binding buffer is formulated for flow cytometric staining of apoptotic cells with conjugated Annexin V. The Annexin V Binding Buffer is recommended for any labeling and washing step using fluorochrome- or biotin-conjugated Annexin V. This buffer contains an optimal concentration of calcium that facilitates the binding of Annexin V to phosphatidylserine on the cell surface. The buffer is provided ready to use.
Applications
Cell Characterization
Size
2 mL
Recommended Use
Annexin V Binding Assays; Apoptosis assays
Area of Interest
Covid-19, Infection, Respiratory infection
Limitation
Restrictions for research use only. Intended for use by laboratory professionals.
Sterility
Sterile
Shelf Life
Stable until expiry date on label. Do not use past expiration date.
Storage
Store at 2 - 8°C. Do not freeze.
Contains
0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution