PharmaOnco™ Erythroid Differentiation Kit

I-O™ Erythroid Differentiation Kit is designed for the Serum-Free (SF) and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to erythroid progenitor cells (erythroblasts) expressing Glycophorin A and CD71.
Use a two-step protocol to differentiate erythroblasts ( Glycophorin A+CD71+) with the high average frequency and high yield. During the first 10 days, I-O™ Hematopoietic Supplement A (3 days) and then Erythroid Supplement E1 (7 days) are added to basal medium to induce cells toward erythroid-biased hematopoietic progenitor cells. These cells are further differentiated into erythroid progenitor cells using Supplement E2 and Hematopoietic Cells Medium II (SF) during the second 14-day stage. Cells generated using I-O™ Erythroid Differentiation Kit can be further matured into normoblasts and reticulocytes when moved to appropriate culture conditions for maturation.
The cells typically expand 51~431-fold during the 14-day erythroid differentiation stage and the Day 24 cell population typically contains 70~80% CD71+GlyA+ erythroblasts.

Serum-Free (SF) formulation

Cell Culture, Differentiation, Expansion

The products in this kit are also available for individual sale. Please contact us for your requirements.

Cells

Immunology, Stem Cell Biology, Cell Therapy Research, Drug Discovery and Toxicity Testing

1 kit

PharmaOnco™ Hematopoietic Basal Medium, 120 mL
PharmaOnco™ Hematopoietic Supplement A, 225 μL
PharmaOnco™ Erythroid Supplement E1 (10X), 10 mL
PharmaOnco™ Erythroid Supplement E2 (10X), 20 mL
PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF), 100 mL

PharmaOnco™ Gentle Cell Dissociation Reagent
PharmaOnco™ hESC/hiPSC Dissociation/Passaging Reagent
PharmaOnco™ Dispase
PharmaOnco™ hESC/hiPSC Maintenance Medium
PharmaOnco™ hESC/hiPSC Maintenance Medium, cGMP
PharmaOnco™ hESC/hiPSC Maintenance Plus Medium, cGMP
Trypan Blue
DMEM/F-12 with 15 mM HEPES
12-well tissue culture-treated plates
96-well flat-bottom plate

Erythroid

Human

Liquid

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Store at -20°C

Stable as supplied until expiry date on label.

Tested for sterility

Restrictions for research use only. Intended for use by laboratory professionals.