PharmaOnco™ Human Memory CD4+ T Cell Isolation Kit

CAT#: ITS-0622-YT405
Product Type: Cell Isolation Kit
Species: Human
Target: CD62L+ cells, unwanted CD45RA+ and non-CD4+ T cells
Description
This kit has been developed to isolate untouched and highly purified CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by positive selection. Isolated cells with up to 94% purity ​are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Desired cells are labeled with antibodies and magnetic particles; Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 1 x 10^9 cells
Target
CD62L+ cells, unwanted CD45RA+ and non-CD4+ T cells
Method Type
Positive Selection, Negative Selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Leukapheresis, PBMC
Size
1 kit
Components
PharmaOnco™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
PharmaOnco™ Dextran Magnetic Beads, 1 mL
PharmaOnco™ Human CD62L Positive Selection Cocktail, 1 mL
PharmaOnco™ Releasable Magnetic Beads, 1 mL
PharmaOnco™ Release Buffer, 1 mL
Material not included
Magnetic separator
Cell Background
T cells
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store at 2 - 8°C.
Do not freeze.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
Shipping
Components may be shipped at room temperature (15 - 25°C). Upon receipt, store it immediately at the temperature recommended below.
For Research Use Only | Not For Clinical Use
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