PharmaOnco™ Human Treg Cell Differentiation Kit

CAT#: ITS-0622-YT368
Product Type: Cell Isolation Kit
Species: Human
Description
PharmaOnco™ Human Treg Cell Differentiation Kit is a set of optimized reagents needed to induce human regulatory T (Treg) cells differentiation. It is designed to generate a highly enriched population of human FoxP3+CD25+ Treg cells.
Applications
Cell Culture, Differentiation, Expansion
Application Notes
This kit provides sufficient reagents for the differentiation of two 24-well plates.
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Size
1 kit
Components
Mouse Anti-Human CD3 Antibody
Human Treg Reagent 1
Human Treg Reagent 2
Human Treg Reagent 3
Human Treg Reagent 4
Reconstitution Buffer 1
Reconstitution Buffer 2
Wash Buffer (20X)
Assay Procedure
Coat a plate with Mouse Anti-Human CD3 Antibody
Isolate naïve CD4+ T cells from Human PBMCs
Culture naïve CD4+ T cells in Human Treg Differentiation Media for 5 days
Verify differentiation into Treg cells by flow cytometry
Cell Background
Regulatory T (Treg) cells
Species
Human
Format
Liquid
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store the unopened product at -20 to -80 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
For Research Use Only | Not For Clinical Use
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