PharmaOnco™ Leukemic Cell Culture Kit

PharmaOnco™ Leukemic Cell Culture Kit have been optimized for culture, expansion, and drug screening of human myeloid leukemia cells. This kit has been tested on chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) samples. The CD34+ CML cells typically expand ~65-fold; the CD34+ AML cells typically expand ~30-fold.

Serum-Free (SF) formulation

Cell Culture, Expansion, Toxicity Assay

The products in this kit are also available for individual sale. Please contact us for your requirements.

Flow Cytometry

Cells

Immunology, Cancer, Stem Cell Biology

1 kit

PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF), 100 mL
PharmaOnco™ CD34+ Expansion Supplement (10X), 10 mL
PharmaOnco™ UM-729, 250 µg

DMSO
Iscove's Modified Dulbecco's Medium (IMDM)
DNase I Solution
Trypan Blue
Tissue culture-treated plates
245 mm x 245 mm Square Treated Tissue Culture Dishes
35 mm Culture Dishes

Use sterile techniques to prepare PharmaOnco™ Leukemic Cell Culture Medium. The following example is for preparing 100 mL of medium. If preparing other volumes, adjust accordingly.
1. Thaw PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF) at room temperature (15 - 25°C) or overnight at 2 - 8°C. Mix thoroughly.
2. Thaw PharmaOnco™ CD34+ Expansion Supplement (10X) at room temperature (15 - 25°C) until just thawed. Mix thoroughly.
3. Add 10 mL of PharmaOnco™ CD34+ Expansion Supplement (10X) to 90 mL of PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF). Mix thoroughly.
4. Add 250 µg of UM-729 to 68 µL of fresh DMSO (final concentration 10 mM).
5. Prepare a 1 in 100 dilution of the 10 mM UM-729 stock solution by performing serial 1 in 10 dilutions.
6. Add 10 µL of diluted UM-729 per mL of medium to give a final concentration of 1.0 µM UM-729. Titration of UM-729 may be required to determine the optimal concentration for CD34+ cell expansion.

Leukemic Cell

Human

Liquid

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF) : Store at -20°C;
PharmaOnco™ CD34+ Expansion Supplement (10X), Store at -20 to -80°C;
PharmaOnco™ UM-729, Store at 15-25°C.

If not used immediately, aliquot into tubes and store at -20°C. After thawing aliquots, use immediately. Do not re-freeze.

Stable as supplied until expiry date on label.

Tested for sterility

Restrictions for research use only. Intended for use by laboratory professionals.