I-O™ Monocyte Differentiation Kit (CAT#: ITS-0622-YT364)

I-O™ Monocyte Differentiation Kit is desighed for the feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to monocytes expressing CD14.
During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14⁺ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14⁺ frequency is typically 60 - 80%.

Serum-Free (SF) formulation

Cell Culture, Differentiation, Expansion

The products in this kit are also available for individual sale. Please contact us for your requirements.

Flow Cytometry

Cells

Immunology, Stem Cell Biology, Cell Therapy Research