PharmaOnco™ Monocyte Differentiation Kit

PharmaOnco™ Monocyte Differentiation Kit is desighed for the feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to monocytes expressing CD14.
During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14⁺ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14⁺ frequency is typically 60 - 80%.

Serum-Free (SF) formulation

Cell Culture, Differentiation, Expansion

The products in this kit are also available for individual sale. Please contact us for your requirements.

Flow Cytometry

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

1 kit

PharmaOnco™ Hematopoietic Basal Medium, 120 mL
PharmaOnco™ Hematopoietic Supplement A (200X), 225 μL
PharmaOnco™ Hematopoietic Supplement B (200X), 225 μL
PharmaOnco™ Monocyte Differentiation Supplement (100X), 3 x 1 mL
PharmaOnco™ CellExpander Hematopoietic Cells Medium II (SF), 3 x 100 mL

PharmaOnco™ Gentle Cell Dissociation Reagent
PharmaOnco™ hESC/hiPSC Dissociation/Passaging Reagent
PharmaOnco™ Dispase
PharmaOnco™ hESC/hiPSC Maintenance Medium
PharmaOnco™ hESC/hiPSC Maintenance Medium, cGMP
PharmaOnco™ hESC/hiPSC Maintenance Plus Medium, cGMP
Trypan Blue
DMEM/F-12 with 15 mM HEPES
6-well tissue culture-treated plates
96-well flat-bottom plate

Monocyte

Human

Liquid

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Store at -20°C

Stable as supplied until expiry date on label.

Tested for sterility

Restrictions for research use only. Intended for use by laboratory professionals.