This kit has been developed to isolate highly purified CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by immunomagnetic negative selection. Isolated cells with up to 96% purity are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 1 x 10^9 cells
Target
non-MDSCs
Method Type
Negative Selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Bone Marrow, Other, Whole Blood
Size
1 kit
Material not included
Magnetic separator
Cell Background
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store at 2 - 8°C. Do not freeze.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboMouseory professionals.