Description
This kit has been developed to isolate highly purified mouse CD19+ B cells from murine splenocytes. The CD19 nano-antibody (CD19 Nb) is a recombinantly expressed low affinity antibody. The CD19 Nb-Strep binds to the matrix of Strep-Agarose columns. Therefore, CD19+ target cells can adhere to the matrix. Finally, the addition of biotin causes the elution of the target cells from the matrix, and Nb-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
Highly pure cells;
Label-free cells
Applications
Cell Isolation
Application Notes
Desired cells are labeled with low affinity CD19 antibody.
Comment
This kit is available for processing 1 x 10^8 cells.
Method Type
Positive selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Murine splenocytes
Datasheet