PharmaOnco™ CellSort Human CD8+ T Cell Isolation Kit, 25 tests

CAT#: ITS-1222-YT47
Product Type: Cell Isolation Kit
Species: Human
Target: Non-CD8+ T cells
Description
This kit has been developed to isolate untouched and highly purified CD8+ T cells from peripheral blood mononuclear cells (PBMCs) by negative selection. Non-CD8+ T cells are depleted by labeling biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoparticles. Isolated cells with 75-87% purity ​are immediately available for downstream applications such as flow cytometry, culture, immune status monitoring, or functional assay.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles.
Comment
This kit is available for processing 1 x 10^9 ells
Target
Non-CD8+ T cells
Method Type
Negative selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
PBMC
Size
1 kit, up to 25 isolations
Components
PharmaOnco™ Human CD8+ T Cell Biotinylated Antibody Cocktail, 1 mL
PharmaOnco™ Buffer, 10 mL
PharmaOnco™ Streptavidin Nanoparticles, 2 mL
Material not included
Magnetic separator
PBS/EDTA Buffer
Fetal bovine serum (FBS)
Tube sealer
Tube
Luer lock syringes
Cell Background
T cells, CD8+
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.
Storage
Store at 2 - 8°C.
Do not freeze.
For Research Use Only | Not For Clinical Use
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