PharmaOnco™ CellSort Human Switched Memory B Cell (CD19+ CD27+ IgD−) Selection Kit

Switched memory B cells, also called CD19 + CD27 + IgD − cells, are indicators of normal B-cell activation and development in germinal centers in lymph nodes or other secondary lymphoid tissues. This kit has been developed to isolate untouched switched memory B cells from human peripheral blood mononuclear cells (PBMCs) by depletion of unwanted cells. Isolated cells ​are immediately available for downstream applications such as flow cytometry, molecular biology and functional assays.

Cell Isolation

Unwanted cells are labeled with antibodies and magnetic particles.

This kit is available for processing 2 x 10^9 cells.

Unwanted cells are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, CD235a (glycophorin A), Anti-IgM, and Anti-IgD antibodies.

Negative selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

PBMCs

1 kit, 20 tests

B Cells

Human

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.