PharmaOnco™ CellSort Mouse CD4+ T Cell Selection Kit, Microbubble Isolation
CAT#: ITS-1222-YT28
Product Type: Cell Isolation Kit
Species: Mouse
Target: Non-CD4+ T cells
Description
This kit has been developed to isolate highly purified CD4+ T cells from mouse spleen by negtive selection. Isolated cells are immediately available for downstream applications such as flow cytometry, phenotypic characterization, culture, or cell-based experiments.
Features
Fast and easy workflow takes only 30 minutes; No magnet, no column, no harsh chemicals.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and depleted.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr-1, TCR‑γδ and TER-119.
Target
Non-CD4+ T cells
Method Type
Negative selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Size
1 kit, 10 tests (∼1x10^8 cells/test)
Cell Background
CD4+ T cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.