Description
This kit has been developed to isolate hf hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow by immunomagnetic positive selection. Lineage committed cells (lineage positive) include T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes are depleted by labeling biotin conjugated antibody cocktail followed by incubation with magnetic nanoparticles. Isolated cells highly enriched for CD117+ cells are immediately available for downstream applications such as flow cytometry, counting, or other applications.
Applications
Cell Isolation
Application Notes
Unwanted cells are removed by immunomagnetic depletion.
Comment
This kit is available for processing 1 x 10^9 cells.
Target
Lineage positive Cell
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse bone marrow
Datasheet