PharmaOnco™ CellSort Two-Step Human Breast Cancer Stem Cell Isolation Kit (CAT#: ITS-1222-YT56)

This kit has been developed to isolate CD24-CD44+ breast cancer stem cells by negative selection and positive selection. Unwanted cells are depleted by labeling biotin conjugated anti-CD24 antibody followed by incubation with magnetic streptavidin nanoparticles, leaving CD24- cells in suspension. Then cells in suspension are labeled with biotinylated anti-CD44 antibody that targets the desired cells. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, mmune statusmonitoring, or other functional assays.​

Cell Isolation

Unwanted cells are labeled with anti-CD24 antibodies and magnetic particles; Desired cells are labeled with anti-CD44 antibodies and magnetic nanoparticles.

This kit is available for processing 1 x 10^9 cells.

CD24- cells, CD44+ cells

Negative selection; positive selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research