PharmaOnco™ CellSort Two-Step Human Breast Cancer Stem Cell Isolation Kit

This kit has been developed to isolate CD24-CD44+ breast cancer stem cells by negative selection and positive selection. Unwanted cells are depleted by labeling biotin conjugated anti-CD24 antibody followed by incubation with magnetic streptavidin nanoparticles, leaving CD24- cells in suspension. Then cells in suspension are labeled with biotinylated anti-CD44 antibody that targets the desired cells. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, mmune statusmonitoring, or other functional assays.​

Cell Isolation

Unwanted cells are labeled with anti-CD24 antibodies and magnetic particles; Desired cells are labeled with anti-CD44 antibodies and magnetic nanoparticles.

This kit is available for processing 1 x 10^9 cells.

CD24- cells, CD44+ cells

Negative selection; positive selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

1 kit

PharmaOnco™ Biotinylated anti-CD24 antibody, 0.5 mL
PharmaOnco™ Biotinylated anti-CD44 antibody, 0.2 mL
PharmaOnco™ Streptavidin Nanoparticles, 2 mL
PharmaOnco™ APC-anti-CD24 antibody, 0.2 mL
PharmaOnco™ PE-anti-CD44 antibody, 0.2 mL
PharmaOnco™ Buffer, 25 mL X 2vials

Magnetic separator

Human Breast Cancer Stem Cell

Human

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.