PharmaOnco™ CellSort Two-Step Human Memory B Cell Selection Kit

CAT#: ITS-1222-YT4
Product Type: Cell Isolation Kit
Species: Human
Target: Step 1: Non B cells; Step 2: CD27+ B cells.
Description
This kit has been developed to isolate highly purified memory B cells from human peripheral blood mononuclear cells (PBMC). The isolation of memory B cells is performed in a two-step procedure, negative selection and positive selection. Isolated cells are available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Applications
Cell Isolation
Application Notes
First step (negative selection): Unwanted cells are labeled with antibodies and deplete; Next step (positive selection): Desired cells are selected with CD27 MicroBeads.
Comment
This kit is available for processing 2 x 10^9 cells.
Target
Step 1: Non B cells; Step 2: CD27+ B cells.
Method Type
Negative selection & positive selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Human PBMC
Size
1 kit
Material not included
Magnetic separator
Cell Background
B Cells
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.
Storage
Store at 2 - 8°C.
Do not freeze.
For Research Use Only | Not For Clinical Use
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