PharmaOnco™ CellSort Two-Step Mouse B Cell Selection Kit for Marginal Zone & Follicular Cells

This kit has been developed to isolate marginal zone B cells and follicular B cells from mouse spleen by two-step procedure. First, the non-B cells, the B-1 cells, and the transitional B cells are deleted to obtain a mixture of marginal zone B cells and follicular B cells. Further step isolates these two subsets by directly labeling CD23(high) follicular cells. Isolated cells ​are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.

Cell Isolation

First step (negative selection): Unwanted cells are labeled with antibodies and deplete; Next step : two subsets cells are separated.

This kit is available for processing 2 x 10^9 cells.

Step 1: Non-B cells, the B-1 cells, and the transitional B cells; Step 2: CD23+ cells.

Negative selection; cell separation

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

Mouse spleen

1 kit, 20 tests

B Cells

Mouse

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.