Semi-solid methylcellulose-based medium for hybridoma selection and cloning, with HAT (serum-containing)
Description
Creative Biolabs provide several clonal selection & culture media and related reagents to accelerate antibody development projects for clients.PharmaOnco™ Hybridoma Selection Medium is a semi-solid medium containing serum and the selective agents hypoxanthine, aminopterin, and thymidine (HAT). It has been verified for use in developing mouse and rat hybridomas.
Features
lot-to-lot consistency; the ease of downstream cell culture/purification of target proteins.
Applications
Cell Culture, Semi-Solid Cloning
Research Areas
Antibody Development, Cancer, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
Size
90 mL
Components
• DMEM • Methylcellulose • Serum • Hypoxanthine, aminopterin, and thymidine (HAT) • Gentamicin • 2-Mercaptoethanol • Phenol red • L-Glutamine and other supplements • Other ingredients
Species
Human, Mouse, Rat, Other
Storage
Store at +2°C to +8°C, protected from light; Once opened store at 4°C and use within 6-8 weeks;