LipidFlux™ AF Cholesterol Assay Kit

CAT#: ITS-1125-HM5
Product Type: Assay Kit
Species: All Species
Short Description
Measures total cholesterol with dual-detection (Colorimetric/Fluorometric).
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ AF Cholesterol Assay Kit utilizes an advanced fluorometric detection system to deliver high-fidelity lipid profiles without the need for thermal activation. This ultra-sensitive assay is critical for investigating the mechanisms of atherosclerosis and screening novel cholesterol-lowering therapeutics in early-stage drug discovery. Its ability to detect low-abundance sterols in micro-volume samples makes it a powerful tool for detailed pharmacodynamic profiling.
Features
Ultra-sensitive dual-readout system eliminates thermal dependency (no 37°C incubation); Optimized for screening lipid-modulating compounds in high-density plate formats.
Process Relevance
Cardiovascular Safety Profiling
Application Stage
Discovery / Basic Research
Applications
High-sensitivity lipid profiling in serum and plasma without a 37°C incubator.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, samples
Research Areas
Cardiovascular Disease; Metabolic Syndrome
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 40 min
LOD
OD 1 mg/dL; FL 0.2 mg/dL
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Cholesterol is a structural component of cell membranes and a precursor for steroid hormones and bile acids. While essential elevated serum levels are a major risk factor for atherosclerosis and cardiovascular disease making its accurate quantification critical in metabolic research.
For Research Use Only | Not For Clinical Use
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