Short Description
Quantifies cellular cholesterol uptake using a fluorescence-based method.
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Cholesterol Uptake Assay Kit provides a safe, non-radioactive method for quantifying cellular cholesterol absorption in drug discovery and metabolic research. Utilizing a fluorescently tagged cholesterol probe, this high-throughput compatible system enables the rapid screening of cholesterol uptake inhibitors and the evaluation of pharmaceutical compounds on lipid metabolism. By offering precise quantification of cholesterol flux in adherent cells, it supports critical studies on cardiovascular health, obesity, and hypercholesterolemia management.
Features
High Sensitivity & Dynamic Range: Utilizes advanced fluorescent labeling to detect minute changes in cholesterol internalization across diverse mammalian cell lines.
Streamlined Homogeneous Format: Simplified "mix-and-read" protocol eliminates complex wash steps, significantly reducing hands-on time and improving experimental reproducibility.
High-Throughput Compatibility: Optimized for 96-well and 384-well microplate formats, ensuring seamless integration into automated robotic screening platforms.
Superior Signal-to-Noise Ratio: Features optimized buffer systems that minimize background interference, providing clear and actionable data for metabolic profiling.
Process Relevance
The kit facilitates high-throughput screening of small molecules to optimize lead compounds targeting dyslipidemia and atherosclerosis.
Application Stage
Ideal for target validation, lead optimization, and pre-clinical potency assessment in cardiometabolic drug development programs.
Applications
The assay enables quantitative analysis of niemann-pick C1-like 1 (NPC1L1) activity, evaluation of statin-mediated uptake changes, and screening of botanical extracts in hepatocyte cultures.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Assesses cellular cholesterol absorption and the activity of specialized transmembrane transport proteins.
Detection Method
Fluorescence Detection (Ex 485 nm / Em 535 nm)
Sample Type
Adherent cells
Research Areas
Cardiovascular disease, atherosclerosis, metabolic syndrome, non-alcoholic fatty liver disease (NAFLD), lipidomics research, etc.
Datasheet