LipidFlux™ Cholesterol Uptake Assay Kit

CAT#: ITS-1125-WB14
Product Type: Assay Kit
Species: All Species
Target: Assesses cellular cholesterol absorption and the activity of specialized transmembrane transport proteins.
Brand: LipidFlux
Development Stage: Research-Grade Lipid Metabolism Assay Development
Short Description
Quantifies cellular cholesterol uptake using a fluorescence-based method.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Cholesterol Uptake Assay Kit provides a safe, non-radioactive method for quantifying cellular cholesterol absorption in drug discovery and metabolic research. Utilizing a fluorescently tagged cholesterol probe, this high-throughput compatible system enables the rapid screening of cholesterol uptake inhibitors and the evaluation of pharmaceutical compounds on lipid metabolism. By offering precise quantification of cholesterol flux in adherent cells, it supports critical studies on cardiovascular health, obesity, and hypercholesterolemia management.
Features
High Sensitivity & Dynamic Range: Utilizes advanced fluorescent labeling to detect minute changes in cholesterol internalization across diverse mammalian cell lines.
Streamlined Homogeneous Format: Simplified "mix-and-read" protocol eliminates complex wash steps, significantly reducing hands-on time and improving experimental reproducibility.
High-Throughput Compatibility: Optimized for 96-well and 384-well microplate formats, ensuring seamless integration into automated robotic screening platforms.
Superior Signal-to-Noise Ratio: Features optimized buffer systems that minimize background interference, providing clear and actionable data for metabolic profiling.
Process Relevance
The kit facilitates high-throughput screening of small molecules to optimize lead compounds targeting dyslipidemia and atherosclerosis.
Application Stage
Ideal for target validation, lead optimization, and pre-clinical potency assessment in cardiometabolic drug development programs.
Applications
The assay enables quantitative analysis of niemann-pick C1-like 1 (NPC1L1) activity, evaluation of statin-mediated uptake changes, and screening of botanical extracts in hepatocyte cultures.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Assesses cellular cholesterol absorption and the activity of specialized transmembrane transport proteins.
Detection Method
Fluorescence Detection (Ex 485 nm / Em 535 nm)
Sample Type
Adherent cells
Research Areas
Cardiovascular disease, atherosclerosis, metabolic syndrome, non-alcoholic fatty liver disease (NAFLD), lipidomics research, etc.
Size
100 tests
Data Output Type
Quantitative fluorescence intensity measurement reflecting the rate and total capacity of cellular lipid internalization.
Assay Time
24-72 h
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Cholesterol homeostasis is critical for membrane integrity and steroidogenesis. Dysregulation of uptake pathways leads to hypercholesterolemia and vascular plaque formation, making absorption mechanisms primary targets for therapeutic intervention.
Pathways
Cholesterol uptake primarily involves the NPC1L1 transporter and clathrin-mediated endocytosis. These processes interact with the SREBP pathway and LXR signaling to maintain intracellular lipid balance and systemic homeostasis.
For Research Use Only | Not For Clinical Use
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