LipidFlux™ Fatty Acid Uptake Assay Kit

CAT#: ITS-1125-WB13
Product Type: Assay Kit
Species: All Species
Target: Transmembrane fatty acid transport proteins (FATPs) and cellular lipid influx mechanisms.
Brand: LipidFlux
Development Stage: Research-Grade Lipid Metabolism Assay Development
Short Description
Quantifies cellular fatty acid uptake using a fluorescence method.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Fatty Acid Uptake Assay Kit offers a non-radioactive, high-throughput solution for quantifying long-chain fatty acid transport in live adherent cells. Utilizing a "mix-and-read" fluorescent format with proprietary quenching technology, it enables rapid screening of fatty acid uptake modulators. This sensitive system is essential for metabolic research and drug discovery targeting obesity, diabetes, and cardiovascular diseases.
Features
Fluorogenic Precision: Employs a high-affinity LCFA analog that yields robust signal-to-noise ratios via bottom-read fluorescence detection.
Homogeneous Workflow: Features a streamlined "add-and-read" protocol that eliminates disruptive cell washing, lysis, or complex staining procedures.
Live-Cell Compatibility: Optimized for real-time kinetic monitoring or endpoint analysis in adherent mammalian cell lines and differentiated adipocytes.
Scalable Architecture: Designed with a stable, quench-based chemistry that is fully compatible with automated liquid handling and high-throughput screening.
Process Relevance
This kit enables precise monitoring of LCFA influx to support metabolic rate modeling and therapeutic lead optimization.
Application Stage
Ideal for secondary drug screening, lead validation, and metabolic process development in preclinical research.
Applications
Quantitative measurement of LCFA transport kinetics in whole cells, characterization of FATP modulators, and screening for drug-induced lipid metabolic disruptions in metabolic research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Transmembrane fatty acid transport proteins (FATPs) and cellular lipid influx mechanisms.
Detection Method
Fluorescence Detection (Ex 488 nm / Em 523 nm)
Sample Type
Adipocytes and other fatty acid-transporting cells. Or chemical compounds
Research Areas
Metabolic disorders, oncology therapeutics, cardiovascular physiology, diabetic nephropathy, etc.
Size
100 Tests
Data Output Type
Quantitative fluorescence intensity measurements representing real-time kinetic uptake or cumulative endpoint accumulation.
Assay Time
2 hrs
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Intracellular fatty acid accumulation is a hallmark of obesity and cardiovascular disease, driven by protein-mediated transport. This kit tracks these vital energetic substrates to elucidate the mechanisms behind ectopic lipid deposition and cellular lipotoxicity.
Pathways
Central to the insulin-stimulated translocation of FATP1 and FATP4, this assay monitors the "vectorial acylation" process. It maps the metabolic flux downstream of PPAR signaling and upstream of mitochondrial β-oxidation.
For Research Use Only | Not For Clinical Use
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