Short Description
Quantifies cellular fatty acid uptake using a fluorescence method.
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Fatty Acid Uptake Assay Kit offers a non-radioactive, high-throughput solution for quantifying long-chain fatty acid transport in live adherent cells. Utilizing a "mix-and-read" fluorescent format with proprietary quenching technology, it enables rapid screening of fatty acid uptake modulators. This sensitive system is essential for metabolic research and drug discovery targeting obesity, diabetes, and cardiovascular diseases.
Features
Fluorogenic Precision: Employs a high-affinity LCFA analog that yields robust signal-to-noise ratios via bottom-read fluorescence detection.
Homogeneous Workflow: Features a streamlined "add-and-read" protocol that eliminates disruptive cell washing, lysis, or complex staining procedures.
Live-Cell Compatibility: Optimized for real-time kinetic monitoring or endpoint analysis in adherent mammalian cell lines and differentiated adipocytes.
Scalable Architecture: Designed with a stable, quench-based chemistry that is fully compatible with automated liquid handling and high-throughput screening.
Process Relevance
This kit enables precise monitoring of LCFA influx to support metabolic rate modeling and therapeutic lead optimization.
Application Stage
Ideal for secondary drug screening, lead validation, and metabolic process development in preclinical research.
Applications
Quantitative measurement of LCFA transport kinetics in whole cells, characterization of FATP modulators, and screening for drug-induced lipid metabolic disruptions in metabolic research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Transmembrane fatty acid transport proteins (FATPs) and cellular lipid influx mechanisms.
Detection Method
Fluorescence Detection (Ex 488 nm / Em 523 nm)
Sample Type
Adipocytes and other fatty acid-transporting cells. Or chemical compounds
Research Areas
Metabolic disorders, oncology therapeutics, cardiovascular physiology, diabetic nephropathy, etc.
Datasheet