LS 174T In Vitro MTS Assay (Cell Viability)

This assay is to provide LS 174T-based In Vitro MTS Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

LS 174T

Human

LS 174T is a cell exhibiting epithelial morphology that was isolated from the colon of a White, 58-year-old, female adenocarcinoma patient with colorectal cancer. This cell line was deposited by Northwestern University and can be used in cancer research.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro MTS Assay (Cell Viability)

LS 174T-cell based In Vitro MTS Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins