MB49 In Vitro Resazurin Assay (Cell Viability)

This assay is to provide MB49-based In Vitro Resazurin Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

MB49

Mouse

MB49 cells are derived from C57BL/Icrf-a' mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed. This abnormality is a frequent early event in human bladder cancer.

Bladder Cancer

Oncology

In Vitro Resazurin Assay (Cell Viability)

MB49-cell based In Vitro Resazurin Assay (Cell Viability)

Resazurin is a non-fluorescent (blue in color) component of alamar Blue reagents, which can enter viable cells. After entering in to viable cells, resazurin is converted to resorufin by NADH, NAD (P) H or FADH2-dependent cellular oxidoreductase.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins