MCF7 In Vitro RNA Expression Analysis Assay (Cap Analysis)

CAT#: ITS-1022-YF1350
Target Cell Organism: Human
Target Cell Alternative Name: MCF-7
Target Cell Name: MCF7
Assay Type: Gene and Protein Expression Assays
Assay Overview
This assay is to provide MCF7-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
MCF7
Target Cell Organism
Human
Target Cell Background
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. MCF7 are epithelial cells isolated from the breast tissue of a 69-year-old, White, female patient with metastatic adenocarcinoma. Use these cells in your breast cancer research.
Target Cell Alternative Name
MCF-7
Related Diseases
Breast Adenocarcinoma
Research Area
Oncology
Assay Name
In Vitro RNA Expression Analysis Assay (Cap Analysis)
Short Description
MCF7-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)
Assay Description
Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression technique has become a useful platform.
Assay Type
Gene and Protein Expression Assays
Assay Type Details
Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.
For Research Use Only | Not For Clinical Use
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