MetaVital™ Mouse Dimethylaniline monooxygenase [N-oxide-forming] 1 (FMO1) ELISA Kit

CAT#: ITS-0326-WB15
Product Type: ELISA Kit
Species: Mouse
Target: Mouse flavin-containing monooxygenase 1 (FMO1)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects mouse FMO1 protein in tissues, plasma, or cell extracts using a sensitive sandwich ELISA assay.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Mouse Dimethylaniline monooxygenase [N-oxide-forming] 1 (FMO1) ELISA Kit enables accurate measurement of FMO1 protein in mouse biological samples. Utilizing high-specificity antibodies in a sandwich ELISA format, this kit supports preclinical studies in drug metabolism, toxicology, and xenobiotic biotransformation research, providing reliable and reproducible quantification of this Phase I metabolic enzyme.
Features
High-affinity, mouse-specific antibody pairs ensure selective detection of FMO1 with minimal cross-reactivity to other flavin-containing monooxygenase isoforms.
Sensitive microplate-based sandwich ELISA enables accurate measurement of low-abundance FMO1 across diverse tissue, plasma, and cellular samples.
Pre-optimized reagents and standardized protocol enhance reproducibility and minimize inter-assay variability in pharmacological and metabolic studies.
Designed for integration into high-throughput preclinical workflows for drug metabolism, enzymology, and toxicology applications.
Process Relevance
Quantifies FMO1 levels to assess Phase I metabolic activity and xenobiotic detoxification in mouse models.
Application Stage
Preclinical pharmacokinetic studies, drug metabolism analysis, toxicology assessment, and metabolic enzyme characterization in mouse models.
Applications
Measurement of FMO1 protein in mouse tissues, plasma, or cultured cells for metabolic enzyme profiling, xenobiotic biotransformation studies, ADME profiling studies, and drug metabolism research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Mouse flavin-containing monooxygenase 1 (FMO1)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), toxicology, pharmacology, enzymology, preclinical biomarker research, xenobiotic metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA detection.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Do not freeze. Keep components away from direct sunlight and avoid prolonged exposure to room temperature during use. Ensure containers are tightly sealed after each use to prevent evaporation or contamination. Return all components promptly to 4°C; storage after handling.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
FMO1 is a cytosolic Phase I enzyme catalyzing N-, S-, and P-oxidation of xenobiotics and endogenous compounds in mice. Its activity modulates metabolic clearance, drug toxicity, and pharmacokinetic behavior, making it critical for preclinical research.
Pathways
FMO1 participates in Phase I oxidative metabolism of xenobiotics, generating more polar metabolites for downstream Phase II conjugation. It complements cytochrome P450 enzymes, contributing to detoxification and modulation of pharmacological effects.
For Research Use Only | Not For Clinical Use
Online Inquiry