ModifiSite™ Chymotrypsin Inhibitor Assay Kit

CAT#: ITS-1125-WB29
Product Type: Assay Kit
Species: All Species
Target: The primary target is chymotrypsin (EC 3.4.21.1) and its associated macromolecular or synthetic inhibitors.
Short Description
Quantifies chymotrypsin inhibitor activity using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Chymotrypsin Inhibitor Assay Kit offers a high-throughput, fluorescence-based platform for screening potential drug candidates and protease inhibitors. Utilizing a quenched FITC-labeled substrate, this homogeneous "mix-incubate-measure" system provides superior sensitivity and a robust Z' factor. Its rapid workflow enables the efficient characterization of inhibitor potency and selectivity in pharmaceutical development.
Features
High Sensitivity: Detects chymotrypsin activity as low as 0.01 mU, ensuring reliable data even in low-abundance biological samples.
Superior Specificity: Employs a unique synthetic fluorogenic substrate that avoids interference from trypsin and other common non-specific endopeptidases.
Streamlined Protocol: Features a simple "mix-incubate-measure" homogeneous format, typically requiring only 1-2 hours for complete high-throughput data acquisition.
Comprehensive Components: Includes all necessary reagents such as specialized assay buffers, activators, and standardized coumarin for immediate kinetic measurement.
Process Relevance
This kit enables precise quantification of inhibitor potency to optimize enzymatic stability and ensure consistency in bioprocessing workflows.
Application Stage
Essential for lead optimization in drug discovery, enzymatic process development, and high-throughput in-process quality control.
Applications
Screening small molecule libraries, profiling natural legume-derived inhibitors, characterizing protease-regulated metabolic pathways, and validating chymotrypsin-targeted therapeutic candidates in cell or tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
The primary target is chymotrypsin (EC 3.4.21.1) and its associated macromolecular or synthetic inhibitors.
Detection Method
Fluorescence Detection (Ex 485 nm / Em 530 nm)
Sample Type
Chemical compounds
Research Areas
Drug discovery (oncology and inflammation), metabolic homeostasis, food science and nutritional antinutrients, pancreatic pathophysiology, etc.
Size
100 tests
Data Output Type
Quantitative kinetic or endpoint fluorescence measurements (Ex/Em = 380/460 nm) representing absolute inhibitory activity.
Assay Time
45 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Chymotrypsin is a critical pancreatic serine protease essential for protein catabolism and metabolic signaling. Dysregulated chymotrypsin activity is implicated in pancreatitis and tumor metastasis, making the study of its inhibitors vital for maintaining physiological homeostatic balance.
Pathways
This enzyme operates within the proteolytic cascade of exocrine pancreatic secretion, converting dietary proteins into absorbable peptides. Its activity is regulated via zymogen activation (chymotrypsinogen) and specific inhibition by Kunitz-type or Bowman-Birk family inhibitors.
For Research Use Only | Not For Clinical Use
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