Short Description
Quantifies chymotrypsin inhibitor activity using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Chymotrypsin Inhibitor Assay Kit offers a high-throughput, fluorescence-based platform for screening potential drug candidates and protease inhibitors. Utilizing a quenched FITC-labeled substrate, this homogeneous "mix-incubate-measure" system provides superior sensitivity and a robust Z' factor. Its rapid workflow enables the efficient characterization of inhibitor potency and selectivity in pharmaceutical development.
Features
High Sensitivity: Detects chymotrypsin activity as low as 0.01 mU, ensuring reliable data even in low-abundance biological samples.
Superior Specificity: Employs a unique synthetic fluorogenic substrate that avoids interference from trypsin and other common non-specific endopeptidases.
Streamlined Protocol: Features a simple "mix-incubate-measure" homogeneous format, typically requiring only 1-2 hours for complete high-throughput data acquisition.
Comprehensive Components: Includes all necessary reagents such as specialized assay buffers, activators, and standardized coumarin for immediate kinetic measurement.
Process Relevance
This kit enables precise quantification of inhibitor potency to optimize enzymatic stability and ensure consistency in bioprocessing workflows.
Application Stage
Essential for lead optimization in drug discovery, enzymatic process development, and high-throughput in-process quality control.
Applications
Screening small molecule libraries, profiling natural legume-derived inhibitors, characterizing protease-regulated metabolic pathways, and validating chymotrypsin-targeted therapeutic candidates in cell or tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
The primary target is chymotrypsin (EC 3.4.21.1) and its associated macromolecular or synthetic inhibitors.
Detection Method
Fluorescence Detection (Ex 485 nm / Em 530 nm)
Sample Type
Chemical compounds
Research Areas
Drug discovery (oncology and inflammation), metabolic homeostasis, food science and nutritional antinutrients, pancreatic pathophysiology, etc.
Datasheet