Short Description
Quantifies total collagen using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Collagen Assay Kit utilizes a specialized colorimetric technique to quantify total collagen content in tissue homogenates and biological fluids. By measuring the concentration of hydroxyproline through chemical oxidation and chromogen development, the system provides a reliable index of connective tissue density. This assay serves as a critical tool for researchers investigating extracellular matrix remodeling, fibrotic pathways, and structural protein synthesis in various research models.
Features
Features a high-sensitivity dye-binding technology that enables rapid, hydrolysis-free collagen quantitation to streamline research in tissue engineering and extracellular matrix remodeling.
Process Relevance
Assessing total collagen synthesis to monitor extracellular matrix formation and structural integrity in vitro.
Application Stage
Tissue engineering, regenerative medicine, and ECM research.
Applications
Determine total collagen content in tissue biopsies and cell culture for investigating extracellular matrix remodeling and fibrosis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 375 nm / Em 465 nm)
Sample Type
Tissue (skin, tendon, lung), cell culture samples, biological fluids.
Research Areas
Extracellular Matrix Research; Fibrosis Studies; Regenerative Medicine
Datasheet