ModifiSite™ Collagen Assay Kit

CAT#: ITS-1125-YL4
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies total collagen using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Collagen Assay Kit utilizes a specialized colorimetric technique to quantify total collagen content in tissue homogenates and biological fluids. By measuring the concentration of hydroxyproline through chemical oxidation and chromogen development, the system provides a reliable index of connective tissue density. This assay serves as a critical tool for researchers investigating extracellular matrix remodeling, fibrotic pathways, and structural protein synthesis in various research models.
Features
Features a high-sensitivity dye-binding technology that enables rapid, hydrolysis-free collagen quantitation to streamline research in tissue engineering and extracellular matrix remodeling.
Process Relevance
Assessing total collagen synthesis to monitor extracellular matrix formation and structural integrity in vitro.
Application Stage
Tissue engineering, regenerative medicine, and ECM research.
Applications
Determine total collagen content in tissue biopsies and cell culture for investigating extracellular matrix remodeling and fibrosis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 375 nm / Em 465 nm)
Sample Type
Tissue (skin, tendon, lung), cell culture samples, biological fluids.
Research Areas
Extracellular Matrix Research; Fibrosis Studies; Regenerative Medicine
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
80 min
LOD
LOD: 2 µg/mL
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
As the most abundant protein in the extracellular matrix, this structural component provides tensile strength and scaffold integrity to various tissues. Its synthesis and degradation are central to tissue remodeling, fibrotic research, and developmental biology.
Pathways
Collagen synthesis is a downstream result of TGF-beta signaling and is central to the structural integrity of the extracellular matrix. It interacts with integrin receptors to mediate cell-adhesion and mechanotransduction pathways.
For Research Use Only | Not For Clinical Use
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