Short Description
Measures alpha-glucosidase activity via p-nitrophenol release.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ α-Glucosidase Assay Kit is a standard screening tool for identifying inhibitors of carbohydrate digestion, a primary mechanism for type 2 diabetes management. This assay validates compounds that delay glucose absorption, supporting the development of therapeutics that blunt post-prandial hyperglycemia. It is also used in research for Pompe disease to assess lysosomal enzyme activity.
Features
Uses specific p-nitrophenol substrate; Standard screen for inhibitors treating type 2 diabetes.
Process Relevance
Diabetes Therapeutic Screening
Application Stage
Hit-to-Lead Screening
Applications
Screening for Pompe disease and carbohydrate metabolism defects.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 405 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Diabetes & Obesity
Datasheet