ModifiSite™ L-Amino Acid Assay Kit

CAT#: ITS-1125-HM34
Product Type: Assay Kit
Species: All Species
Short Description
Measures total L-amino acid content.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ L-Amino Acid Assay Kit provides a global assessment of the free L-amino acid pool, useful for monitoring cell culture health and evaluating nutritional interventions. This assay is widely used to assess proteolysis rates in cell health assays and to optimize formulation strategies for amino acid supplements. It offers a rapid method for determining the overall nutritional status of biological samples in pre-clinical studies.
Features
Measures total free L-amino acid pool; Evaluates nutritional support formulations and proteolysis rates in cell health assays.
Process Relevance
Cell Culture Media Optimization
Application Stage
Manufacturing (Upstream)
Applications
General assessment of nutritional status and proteolysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, culture media, tissue homogenates, cell lysates, urine, food samples, samples
Research Areas
Nutrition; Bioprocessing
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 60 min
LOD
3.3 µM (Colorimetric assay, OD); 0.13 µM (Fluorimetric assay, FL)
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
L-Amino acids are the building blocks of proteins and precursors for many biologically active molecules. Monitoring total amino acid pools is vital for assessing nutritional status and optimizing cell culture conditions.
For Research Use Only | Not For Clinical Use
Online Inquiry