Short Description
Determines Phospholipase D activity by measuring choline release.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Phospholipase D Assay Kit allows for the continuous monitoring of enzyme activity, facilitating the study of lipid signaling pathways involved in cell proliferation and migration. This tool is vital for validating PLD inhibitors as potential therapeutics for cancer and inflammatory diseases. By quantifying choline release, it provides kinetic data necessary for understanding membrane remodeling and signal transduction mechanisms.
Features
Continuous spectrophotometric monitoring allows for kinetic analysis; Validates inhibitors of lipid signaling pathways.
Process Relevance
Signal Transduction Research
Application Stage
Target Validation
Applications
Studying lipid signaling and membrane remodeling in cell biology.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Signal Transduction; Oncology
Datasheet