ModifiSite™ Phospholipase D Assay Kit

CAT#: ITS-1125-HM43
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Determines Phospholipase D activity by measuring choline release.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Phospholipase D Assay Kit allows for the continuous monitoring of enzyme activity, facilitating the study of lipid signaling pathways involved in cell proliferation and migration. This tool is vital for validating PLD inhibitors as potential therapeutics for cancer and inflammatory diseases. By quantifying choline release, it provides kinetic data necessary for understanding membrane remodeling and signal transduction mechanisms.
Features
Continuous spectrophotometric monitoring allows for kinetic analysis; Validates inhibitors of lipid signaling pathways.
Process Relevance
Signal Transduction Research
Application Stage
Target Validation
Applications
Studying lipid signaling and membrane remodeling in cell biology.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Signal Transduction; Oncology
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.04 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Phospholipase D enzymes hydrolyze phospholipids to generate phosphatidic acid. Phosphatidic acid is a key lipid second messenger involved in vesicle trafficking cell proliferation and signal transduction.
For Research Use Only | Not For Clinical Use
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