ModifiSite™ Protein Assay Kit

CAT#: ITS-1125-YL29
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Cell-Based Assay Development
Short Description
Measures protein using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Cell-Based Assay Development
Description
This ModifiSite™ Protein Assay Kit utilizes a specialized colorimetric method for the precise quantification of protein concentrations in the presence of challenging detergents and reducing agents. The system features an improved formulation that ensures high stability and sensitivity compared to traditional methods. This reliable tool facilitates research in membrane protein analysis and cell signaling, offering a streamlined workflow for accurate quantification in diverse biochemical research environments.
Features
Utilizes a high-affinity protein-binding dye for ultra-sensitive quantification in dilute samples, providing a reliable tool for characterizing low-yield protein fractions and purified biomolecules.
Process Relevance
Measuring protein levels to assess biomass production and optimize protein purification workflows in research.
Application Stage
Bioprocess development and protein formulation research.
Applications
Measure protein concentrations in samples with limited volume using a micro-scale colorimetric format for precise biochemical analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 360 nm / Em 450 nm)
Sample Type
Cell lysates, serum, plasma, biological fluids.
Research Areas
Proteomics; Molecular Biology; Biochemistry
Size
200 tests
Data Output Type
Quantitative concentration measurement
Assay Time
5 min
LOD
LOD: 1.3 µg/mL
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Kinetic protein assays allow for the real-time monitoring of protein concentration and binding events. These methods are particularly useful for studying dynamic biochemical processes and optimizing reaction conditions.
Pathways
Protein carbonylation is a downstream consequence of ROS-mediated oxidation of amino acid side chains. It often serves as a signal for protein misfolding and subsequent degradation via the proteasomal pathway.
For Research Use Only | Not For Clinical Use
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